Troubleshooting low ESI sensitivity on QTofs with MagneTOF detectors (Guided Troubleshooting) - WKB101295
Article number: 101295
SYMPTOMS
- Low sensitivity when directly infusing a sample or when injecting an LCMS sample or both
- Low resolution observed in some cases
ENVIRONMENT
- Xevo G2
- Xevo G2-S
- Xevo G2-XS
- SYNAPT G2
- SYNAPT G2-S
- SYNAPT G2-Si
STRATEGY
SOFTWARE SOLUTIONS
- Verify that the Tune page settings are correct:
a. Check that Veff is a normal value and adjust as needed - WKB51479
b. Check the quad profile and set to auto profile or the setting that is normally used - WKB91752
c. Check that the TriWave region gas flow settings are at defaults - WKB63006 - If sensitivity decreases over the course of a batch, enable automatic detector gain control - WKB62583
- Optimize the collector voltage - WKB99046
HARDWARE SOLUTIONS
- Check the argon supply. Open tank, adjust regulator, or replace tank as required - WKB886
- If sensitivity is low when directly infusing a sample, ensure that the sample is in the correct reservoir position - WKB16264
- Check the sample probe position and optimize as necessary - WKB7084
- Reboot electronics - WKB93040
ADDITIONAL INFORMATION
If high background/contamination peaks from the LC are observed, use higher-quality solvents (e.g., Waters solvent kit, MilliQ water, LCMS-grade solvents, etc.) - WKB73035
id101295, Electrospray, MALDIG2SI, SYNG2HD, SYNG2HDMAL, SYNG2MS, SYNG2MSMAL, SYNG2SHD, SYNG2SHDML, SYNG2SIHD, SYNG2SIMS, SYNG2SMS, SYNG2SMSML, XEVOG2QTOF, XEVOG2SQTF, XEVOG2STOF, XEVOG2TOF, XEVOG2XSTF