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Large artifact peak, no real signal on QToF instrument - WKB51479

Article number: 51479

SYMPTOMS

  • Large artifact Peak at 286 Da in Negative Resolution mode
  • Peak is visible in Operate and Source Standby
  • All other modes have normal signal
  • Low-mass peaks are still visible when zoomed in and have the proper resolution and isotopic pattern

ENVIRONMENT

  • SYNAPT G2-Si
  • Xevo G2-XS QTof

CAUSE

Veff value in Acquisition Settings was reset to 1000.

FIX or WORKAROUND

  1. Copy the Veff value from Negative Sensitivity mode.
  2. Fine-tune the Veff value to give the correct mass for leucine enkephalin.

ADDITIONAL INFORMATION

Correct settings:

good settings.JPG

 

The m/z of the artifact peak is dependent on the Veff value setting. On the Xevo G2-XS, Veff was set to 1700 and the artifact was 374.5. Set the Veff to whatever settings work in the working mode for the same polarity.

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