Pressure fluctuation on Alliance HPLC System (Guided Troubleshooting) - WKB96127

SYMPTOMS

• Pressure fluctuation on Alliance HPLC System

ENVIRONMENT

• Alliance HPLC System
• 2695 Separations Module
• 2695D Dissolution Separations Module
• 2795 Separations Module

STRATEGY

1. Ensure that the Stroke Volume setting is appropriate for the flow range. WKB25194
2. Check the lines for air bubbles and degas the mobile phase. WKB7030
3. Check the solvent bottles for microbial growth. WKB57411
4. Verify that the solvent filters and solvent sinkers are not clogged. WKB45863
5. Check if check valves are sticky after switching solvents. WKB2141
6. Check for faulty check valves. WKB10722
7. Check for blockage in the inline filter. WKB69397
8. Check for clogged inlet tubing. WKB83547
9. Check for leak at pump heads. WKB18122
10. Inspect the pump head assembly and the seal wash assembly. WKB54901

ADDITIONAL INFORMATION