Alliance pressure fluctuation or no flow - WKB7030

SYMPTOMS

• Pressure fluctuations observed after changing mobile phase
  • Pressure fluctuations between 100 psi and 700 psi at 0.3 mL/min in 100% water
• Several wet prime cycles, no air bubbles
• V3 purge outlet clear waste line does not have a steady stream of flow during prime
• Water in line A and B
• Acetonitrile in lines C and D
• Early retention times

ENVIRONMENT

• 2695 Separations Module
• 2795 Separations Module

CAUSE

Air bubbles in lines, loss of prime in solvent manager.

FIX or WORKAROUND

Priming the solvent manager:

1. Perform a manual dry prime using a syringe to draw mobile phase from each line.
2. Perform a wet prime on each solvent management mobile phase line.
3. Operate flow rate and confirm pressure stability.
4. If pressure fluctuations continue:
   • Repeat steps using methanol to remove all air in the lines.
   • Consider wet priming one additional step using 25%A, 25%B, 25%C, 25%D composition each line.

ADDITIONAL INFORMATION

For more information, refer to the Waters Alliance e2695 Separations Module Operators Guide.