Error: Bubble found on compression on my 2695 - WKB2141
Article number: 2141
SYMPTOMS
- Bouncing or fluctuating pressure
- Little to no flow observed during and after priming
ENVIRONMENT
- Alliance HPLC System
- 2695 Separations Module
- 2795 Separations Module
CAUSE
Switching from a buffered mobile phase using a higher composition of organic than the original buffer causes the check valve to stick.
FIX or WORKAROUND
- Use a higher aqueous wash.
- Sonicate check valves. To avoid solvent leakage, first close the GPV. From the main screen, select Diagnostics > Other Tests > Turn Off GPV.
- Sonicate check valves in water for 15 minutes.
- Sonicate in methanol or acetonitrile for an additional 15 minutes.
- Reinstall check valves.
- Reset GPV and select OK.
- Prime unit at 7.5 ml for 3-5 minutes.
- Monitor flow and pressure. If resolved, continue with analysis.
ADDITIONAL INFORMATION
Replacing a Check Valve Cartridge
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