Troubleshooting BioAccord instrument setup failures (Guided Troubleshooting) - WKB95208

SYMPTOMS

• Instrument setup won't run
• Instrument setup fails
• Calibration fails
• Manually infusing lockmass solution yields weak or no signal 
• "Low Response Detected" error message

ENVIRONMENT

• BioAccord System
• ACQUITY RDa Detector

STRATEGY

1. Make sure to use the correct LockSpray solution - WKB119274
2. If setup won't run, ensure that your vacuum level is <7e-7 mbar - WKB72092
3. Ensure that fluidics solutions are not empty and that the fluidics tubing is fully submerged in the fluidics solutions - WKB53359
4. Purge the syringe a few times - WKB53253
5. If some calibration peaks are missing, sonicate the calibration solution - WKB72090
6. Depending on which step failed:
   1. Make fresh lockmass - WKB70324
   2. Make fresh calibrant - WKB53365

ADDITIONAL INFORMATION