Insufficient sensitivity on a Xevo Mass Spectrometer - WKB17847

SYMPTOMS

• Sensitivity is too low to quantitate on a sample being set up for the first time
• Low sensitivity after a PM

ENVIRONMENT

• Xevo TQD
• SQD2
• Xevo TQ-MS
• Xevo TQ-S
• Xevo TQ-S micro
• Xevo TQ-XS

CAUSE

The wrong resolution mode is being used.

FIX or WORKAROUND

1. Open the console and go to Xevo TQD > IntelliStart.
2. Select either Quantitative or Low Resolution for the resolution mode.
3. Ensure that the resolution mode is calibrated and the tune page has the correct LM/HM resolution and ion energy for that mode.
4. Link the calibration to the tune page being used, or better yet, run a sample tune and development to get a tune page with the correct resolution settings and calibration linked.

ADDITIONAL INFORMATION

• Quantitative resolution has MS1 with 0.75 Da wide peaks and MS 2 with 1 Da wide peaks.
• Low Resolution has MS1 with 1 Da wide peaks and MS 2 with 1 Da wide peaks.
• The wider the peaks (lower resolution), the greater the sensitivity.