Poor QTof resolution on intact proteins or monoclonal antibodies (mAb) - WKB115197

SYMPTOMS

• Poor resolution on intact proteins or monoclonal antibodies

ENVIRONMENT

• Xevo QTof
• Xevo G2 QTof
• Xevo G2-S
• Xevo G2-XS
• Synapt HDMS
• SYNAPT G2-S 
• SYNAPT G2-Si
• Vion IMS QTof
• ACQUITY RDa
• BioAccord

CAUSE

Salt residues in source - The presence of Na or K creates adducts, resulting in a complicated spectrum.

FIX or WORKAROUND

Replace the sample cone with a new one.

ADDITIONAL INFORMATION

As an alternative action, increasing the cone gas flow rate may reduce the negative effect on the intact protein envelop. See the related KCS article WKB117940.