Excessive TFA adducts seen on intact protein mass spectra - WKB117940
Article number: 117940
SYMPTOMS
- TFA adducts affect the resolution of intact protein envelop
ENVIRONMENT
- Trifuoroacetic acid
- Intact protein 60–80 kDa
- ACQUITY UPLC H-Class
- ACQUITY UPLC I-Class
- Xevo G2-XS
CAUSE
Likely cause is a dirty sample cone.
FIX or WORKAROUND
From the MS tune page source settings, adjusting the cone gas from 50 L/hour to 200 L/hour should decrease the adduct level in the MS data.
ADDITIONAL INFORMATION
id117940, monoclonal antibody, UPIBSM, UPIBSM+, UPISMFL, UPISMFL+, UPISMFTIVD, UPISMFTN, UPISMFTN+, UPQSM, UPQSM+, UPQSMBIO, UPQSMBIO+, UPSMFTN, UPSMFTN+, UPSMFTNB+, UPSMFTNBIO, XEVOTQ, XEVOTQA, XEVOTQIVD