How is the gradient curve (composition) set when the curve is set in the inlet method? - WKB99596

ENVIRONMENT

• MassLynx
• Empower
• Alliance HPLC System
• ACQUITY UPLC
• ACQUITY UPLC I-Class
• ACQUITY UPLC H-Class

ANSWER

%B = IC + (FC – IC)(t/T) m

IC: Initial condition

FC: Final condition (in the gradient interval)

T: End time of the gradient interval

t: Gradient elapsed time

m: Curve number       6 = X               5 = X^1/2

                            7 = X²             4 = X^1/3

                            8 = X³             3 = X^1/4

                            9 = X⁴             2 = X^1/5

                            10 = X⁵           (1 and 11 are stepwise)

ADDITIONAL INFORMATION