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TUV/PDA detectors: High background noise and drift baseline- WKB9482

Article number: 9482

SYMPTOMS

  • High background noise
  • Drift baseline stepping upward
  • Peaks very low (compared to earlier chromatograms on the same system) 
  • Lamp is new or it has less than 2000 hours
  • Read Energy at 230 nm (water running through flow cell) <5000
  • Noise SQT fails
  • Dynamic leak test passes
  • Fresh mobile phases
  • New column and pre-column
  • Noise and drift test fails

ENVIRONMENT

  • ACQUITY UPLC PDA Detector
  • ACQUITY UPLC Tunable UV Detector
  • ACQUITY Arc 2998
  • 2487 Dual-Wavelength Absorbance Detector
  • 2489 UV/Vis Detector
  • 2996 PDA Detector
  • 2998 Photodiode Array Detector

CAUSE

  • Dirty flow cell

FIX or WORKAROUND

  1. Flush the flow cell according to "ACQUITY UPLC TUV Detector Operator’s Overview and Maintenance Guide" (from page 40)*
  2. If issue persists, replace the flow cell (see how to replace the flow cell on "ACQUITY UPLC TUV Detector Operator’s Overview and Maintenance Guide", from page 45)

ADDITIONAL INFORMATION

*Flush all channels. Cleaning solution must not go through an analytical column or the Mass Spectrometry (MS) equipment. Remove the MS and analytical column from the flow path before flushing. Set low flow rate and clean the System overnight. Thereafter, prime the system with standard solvents to waste. Run the system overnight with a low-flow method to flush away the cleaning solution.

eluent

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