2695 Separations Module System Cleaning - WKB91424

OBJECTIVE or GOAL

• 2695 Alliance HPLC Separations Module System Cleaning

ENVIRONMENT

• 2695 Separations Module System
• Alliance HPLC System
• 2695 Separations Module

PROCEDURE

Cleaning procedures
1. Prepare the washing solvents (three solutions needed: 100% of water , Acetonitrile:Isopropanol:Methanol:Water =  25:25:25:25, and 30% phosphoric acid in water)
2. Add each one of the washing solvents into sample vials
3. Remove the column and connect the 2695 injector outlet tube to the detector inlet tube with a union (If the detector does not need cleaning, do not connect)
4. Remove the solvent filter, only during the acid wash stage.
5. Dry prime and run System Prep with all lines with the washing solvent, except the seal wash line (100% water).
6. Create the instrument method used for cleaning:

• A/B/C/D = 25/25/25/25
• Flow rate: 0.5mL/min
• Lamp OFF
• Collect 2695 Pressure Trace

7. Create the sample set used for cleaning:

• Injection volume: 100μL
• Run Time: 0.1 min
• Number of Injections: 30

*For the above cleaning steps:

• Start with all lines in 100% water, prime, run sample set and continue to wash with water for a total of 30 minutes to an 1 hour.
• Then with all lines in Acetonitrile:Isopropanol:Methanol:Water =  25:25:25:25 prime, run sample set and continue to wash with for a total of 1-2 hours.
• Then with all lines in 100% water, prime, run sample set and continue to wash with water for a total of 30 minutes.
• Then with all lines in 30% phosphoric acid, remove solvent filters, prime, run sample set and continue to wash with for a total of 1-2 hours.
• Then with all lines in 100% water, prime, run sample set and continue to wash with water for a total of 4x as long as the acid step. (specified time is a reference).

ADDITIONAL INFORMATION

Carryover solution that can be performed by user with 2695 Separations Module - WKB88700