Saturated peaks on FLR detector - WKB94394
Article number: 94394
SYMPTOMS
- Saturated peaks on the chromatogram
ENVIRONMENT
- ACQUITY UPLC FLR Detector
- 2475 Multi-Wavelength Fluorescence Detector
CAUSE
The detector was normalized in a high concentration of organic mobile phase.
The Raman gain was >1000 after the Emission unit normalization.
FIX or WORKAROUND
Repeat the normalization process in 100% water.
How to perform the ACQUITY UPLC FLR detector emission units normalization test - WKB238324
How to normalize emission units with 2475 Fluorescence Detector - WKB193270
ADDITIONAL INFORMATION