ACQUITY UPLC SM-FTN shows variable RSD - WKB86411
Article number: 86411
SYMPTOMS
- High and variable RSD when injecting standards
- System leak test fails when including the needle and needle seal
ENVIRONMENT
- ACQUITY UPLC Sample Manager
- ACQUITY UPLC H-Class
- ACQUITY UPLC I-Class
CAUSE
A leak between the needle and the needle seal causes variable injection responses and a high RSD.
FIX or WORKAROUND
Replace the needle and the needle seal
Needle seals and guides are included in the following kits (15 µL is the standard needle volume)
- 15 µL SS needle (H-Class): 700005215
- 15 µL textured needle (H-Class PLUS & I-Class PLUS): 700011628
- 15 µL MP35N needle (H-Class Bio): 700005421
- 15 µL MP35N textured (H-Class Bio PLUS): 700011658
- 30 µL SS needle (H-Class & I-Class): 700005279
- 30 µL textured needle (H-Class PLUS & I-Class PLUS): 700011698
- 15 µL SS needle (I-Class): 700008977
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Recalibrate the needle Z-axis