ACQUITY UPLC SM-FTN shows variable RSD - WKB86411

SYMPTOMS

• High and variable RSD when injecting standards
• System leak test fails when including the needle and needle seal

ENVIRONMENT

• ACQUITY UPLC Sample Manager 
• ACQUITY UPLC H-Class
• ACQUITY UPLC I-Class

CAUSE

A leak between the needle and the needle seal causes variable injection responses and a high RSD.

FIX or WORKAROUND

Replace the needle and the needle seal

Needle seals and guides are included in the following kits (15 µL is the standard needle volume)

• 15 µL SS needle (H-Class): 700005215
• 15 µL textured needle (H-Class PLUS & I-Class PLUS): 700011628
• 15 µL MP35N needle (H-Class Bio): 700005421
• 15 µL MP35N textured (H-Class Bio PLUS): 700011658
• 30 µL SS needle (H-Class & I-Class): 700005279
• 30 µL textured needle (H-Class PLUS & I-Class PLUS): 700011698
• 15 µL SS needle (I-Class): 700008977

• Recalibrate the needle Z-axis

ADDITIONAL INFORMATION

Video How to replace the sample needle on an ACQUITY Flow Through Needle (FTN) Sample Manager

Article How to replace the sample needle on an ACQUITY Flow Through Needle (FTN) Sample Manager - WKB7319