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"Sample loop calibration out of range" and "Needle calibration out of range" error messages occur when characterizing the needle and loop volume - WKB8430

Article number: 8430

SYMPTOMS

  • After changing the loop from 5 µL to 10 µL and changing the needle, "Loop calibration out of range" and "Needle calibration out of range" error messages occur when trying to characterize the needle and loop volume
  • Priming the system does not resolve the issue 
  • Power-cycling the sample manager does not resolve the issue 
  • The sample loop, syringe, and needle are configured correctly in the console
  • No leaks are present in the system after changing the needle and sample loop 

ENVIRONMENT

  • ACQUITY UPLC Sample Manager

CAUSE

The volume detection device is not calibrated.

FIX or WORKAROUND

  1. Recalibrate the volume detection device.
  2. Perform wash cycles.
  3. Characterize the needle and loop volume.

ADDITIONAL INFORMATION

 

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