Skip to main content
Waters

What is the difference between the low dispersion flow cell and the analytical flow cell on the ACQUITY TUV and PDA Detector? - WKB1187

Article number: 1187

ENVIRONMENT

  • ACQUITY UPLC
  • ACQUITY UPLC H-Class
  • ACQUITY UPLC I-Class
  • ACQUITY TUV
  • ACQUITY PDA

ANSWER

  • The analytical flow cell has an inlet and outlet tubing ID of .005".
  • The low dispersion flow cell (standard cell for I-Class Systems) has an inlet tubing ID of .0025" and an outlet tubing ID of .0035".
  • The 500nL flow cells in the table below may be identified by their inlet and outlet tubing:
    • .005" tubing is red PEEK
    • .0025" tubing is tan (natural) PEEK

 

Part Number Detector Description of Change Previous Name New Name
205015033 TUV

This is the current standard flow cell in all ACQ-TUVs.
It will be renamed and be the standard flow cell in the I-Class TUV only.

Analytical Low Dispersion Analytical
205015016 TUV This part number will be the standard flow cell in the standard ACQ-TUV. Analytical Analytical
205015032 TUV This is the optional 250nL flow cell for the ACQ-TUV.
The name will be changed to avoid confusion with the 500nL flow cell.
Low Dispersion Low Dispersion Microbore
205015036 PDA

This is the current standard flow cell in all ACQ-PDAs.
It will be renamed and be the standard flow cell in the I-Class PDAs only.

Analytical Low Dispersion Analytical
205015017 PDA This part number will be the standard flow cell in the standard ACQ-PDAs. Analytical Analytical
2050150035 PDA

This is the optional 250nL flow cell for the ACQ-PDA.
The name will be changed to avoid confusion with the 500nL flow cell.

Low Dispersion Low Dispersion Microbore

 

Tubing ID Differences and Volumes of Flow Cells

 

Flow Cell Path length Teflon AF ID Inlet ID  Outlet ID Inlet Tube Volume (nL) Optical Volume (nL) Outlet Tube Volume (nL) Total Volume (nL)
Current Analytical 10mm 240µm .005" .005" 1078 448 1207 2733
Low Disp Analytical 10mm 240µm .0025" .0035" 270 448 591 1309
New 250nL 10mm 183µm .0025" .0025" 270 263 302 835

 

 

ADDITIONAL INFORMATION

  • Low dispersion cell and the standard analytical cells have different tubing from the bulkhead connections to the cell body.
  • The lower volume tubing provides less dispersion when the detector is in series before another detector or mass spectrometer.
  • You see the primary advantage of using the low dispersion flow cell if another detector is upstream.

UPAPDA, UPBINARY, UPC2PDA, UPIBSM, UPIBSM+, UPISMFL, UPISMFL+, UPISMFTIVD, UPISMFTN, UPISMFTN+, UPPDA, UPPDAARC, UPPDAARCB, UPPDA-E, UPPDA-L, UPPDALTC, UPPDATC, UPPPDA, UPPTUV, UPQSM, UPQSM+, UPQSMBIO, UPQSMBIO+, UPSMFTN, UPSMFTN+, UPSMFTNB+, UPSMFTNBIO, UPSMPMGR, UPTUV, UPTUVARC, UPTUVARCB, UPTUV-E, UPTUVTC

Not able to find a solution? Click here to request help.