What is the difference between the low dispersion flow cell and the analytical flow cell on the ACQUITY TUV and PDA Detector? - WKB1187
Article number: 1187
ENVIRONMENT
- ACQUITY UPLC
- ACQUITY UPLC H-Class
- ACQUITY UPLC I-Class
- ACQUITY TUV
- ACQUITY PDA
ANSWER
- The analytical flow cell has an inlet and outlet tubing ID of .005".
- The low dispersion flow cell (standard cell for I-Class Systems) has an inlet tubing ID of .0025" and an outlet tubing ID of .0035".
- The 500nL flow cells in the table below may be identified by their inlet and outlet tubing:
- .005" tubing is red PEEK
- .0025" tubing is tan (natural) PEEK
| Part Number | Detector | Description of Change | Previous Name | New Name |
|---|---|---|---|---|
| 205015033 | TUV |
This is the current standard flow cell in all ACQ-TUVs. |
Analytical | Low Dispersion Analytical |
| 205015016 | TUV | This part number will be the standard flow cell in the standard ACQ-TUV. | Analytical | Analytical |
| 205015032 | TUV | This is the optional 250nL flow cell for the ACQ-TUV. The name will be changed to avoid confusion with the 500nL flow cell. |
Low Dispersion | Low Dispersion Microbore |
| 205015036 | PDA |
This is the current standard flow cell in all ACQ-PDAs. |
Analytical | Low Dispersion Analytical |
| 205015017 | PDA | This part number will be the standard flow cell in the standard ACQ-PDAs. | Analytical | Analytical |
| 2050150035 | PDA |
This is the optional 250nL flow cell for the ACQ-PDA. |
Low Dispersion | Low Dispersion Microbore |
Tubing ID Differences and Volumes of Flow Cells
| Flow Cell | Path length | Teflon AF ID | Inlet ID | Outlet ID | Inlet Tube Volume (nL) | Optical Volume (nL) | Outlet Tube Volume (nL) | Total Volume (nL) |
|---|---|---|---|---|---|---|---|---|
| Current Analytical | 10mm | 240µm | .005" | .005" | 1078 | 448 | 1207 | 2733 |
| Low Disp Analytical | 10mm | 240µm | .0025" | .0035" | 270 | 448 | 591 | 1309 |
| New 250nL | 10mm | 183µm | .0025" | .0025" | 270 | 263 | 302 | 835 |
ADDITIONAL INFORMATION
- Low dispersion cell and the standard analytical cells have different tubing from the bulkhead connections to the cell body.
- The lower volume tubing provides less dispersion when the detector is in series before another detector or mass spectrometer.
- You see the primary advantage of using the low dispersion flow cell if another detector is upstream.