2695 Separations Module: Peak retention time delayed, but returned in the next run - WKB101785

SYMPTOMS

• Peak retention time delayed, but returned in the next run

ENVIRONMENT

• 2695 Separations Module

CAUSE

Pump flow differs from the set value

FIX or WORKAROUND

1. Acquire pressure fluctuations as data and confirm the pressure fluctuation when the same issue occurs next time.
   In Empower, how can I check system pressure on an Alliance 2695 after a run is completed? - WKB2879
   
2. There may be a problem with the check valve, clean or replace it to see if any improvements.
   How to clean check valves on a 2695 Separations Module Alliance and ACQUITY UPLC- WKB5864
   Video - How to replace the 2695 check valve
3. Check the flow rate with a graduated cylinder at each of the column inlet and detector outlet (There is a possibility that the flow rate seems normal because there is not always an abnormality)

ADDITIONAL INFORMATION