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How to do Component-based Expected Mass Processing - WKB68226

Article number: 68226

OBJECTIVE or GOAL

Welcome back to Get Empowered! In the last Empower tip-of-the-week post for Empower Chromatography Data Software, we learned how to display MS spectra from a particular MS scan along with UV spectra in the Mass Analysis window (Tip #115).

In this week’s tip, we will learn about Expected Mass Processing.

Empower provides two ways to determine if a specified mass exists in a sample. You may be looking for a specific mass because it identifies a desired ‘component’ or an ‘impurity’ in the sample. Today, we will look at ‘component-based’ Expected Mass Processing.

Let’s get started.

ENVIRONMENT

  • Empower

PROCEDURE

  1. Bring the Sample Set (or injections) into Review and open and apply the Method Set. Go to the Processing Method and click the ‘MS Expected Mass’ tab.
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  1. Select ‘Component-based’ processing and enter the Expected Mass or Masses along with the Expected Intensities. In this case, the compound of interest has a molecular ion with a base peak of 621, a fragment ion with mass of 603, a sodium adduct ion with mass of 643, and isotopic ions with masses of 622 and 644. The Peak Separation has a default value of 1 and sets the range over which Empower looks for the expected mass. For example, for mass 621 the range would be 620.5 to 621.5. The expected intensity is a % of the base peak and if the % is equal to or higher than the entered value it is acceptable.
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  1. In this example, all expected masses are ‘found’ and the expected intensities are ‘acceptable’.
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  1. In this example, some of the expected masses are ‘not found’ and the expected intensities are ‘below the value’ entered in the Processing Method, therefore, Empower flags the peak.
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It’s that easy!

ADDITIONAL INFORMATION

  • This procedure can be followed using the QuickStart or Pro interface.
  • ACQUITY QDa Mass Detector is compatible with the Alliance HPLC system.

EMP2LIC, EMP2OPT, EMP2SW, EMP3GC, EMP3LIC, EMP3OPT, EMP3SW, EMPGC, EMPGPC, EMPLIC, EMPOWER2, EMPOWER3, EMPSW, SUP

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