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How to display spectra from a particular MS scan along with UV spectra in the Mass Analysis window - Tip115

Article number: 60852

OBJECTIVE or GOAL

Welcome back to Get Empowered! In the last Empower tip-of-the-week, we learned how to use a library of MS spectra to identify spectra from unknown peaks via the automated technique.
Today I would like to answer a question which I have received from several readers. If I have collected multiple 3D scans with my QDa how do I display spectra from a particular MS scan along with my UV spectra in the Mass Analysis window?  
Let’s see how it’s done!
 

ENVIRONMENT

  • Empower

PROCEDURE

  1. We start in the Main Window of Review and see the PDA scan and the 2 MS scans listed in the 3D Channels table.

clipboard_ebe7db80d635aa2b48a1ff2e8df310d17.png

  1. In the Mass Analysis Window we see the UV spectra and MS spectra from QDa 1 scan.

clipboard_e167fae95a25b20b551148fd6c0f18e2d.png

  1. Go to the Processing Method and select the MS 3D Channel tab.  Towards the bottom of the window you can select which MS scan you would like to use on a peak by peak basis from the 3D Channel Name (Description) field.

clipboard_e38e1cad5e97c84ada159c9abbe249e80.png

  1. Reapply the Method Set and we now see the UV spectra and the MS spectra from QDa 2 scan in the Mass Analysis window.

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It’s that easy!

ADDITIONAL INFORMATION

Final Notes

  • This procedure can be followed using the QuickStart or Pro interface. 
  • For information on Empower training please go to www.waters.com/training and select Empower from the Topic menu. 
     

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