HDX: How to filter solvents and buffers - WKB5268

OBJECTIVE or GOAL

Filtered solvents are less likely to clog up valves and pumps at low flow rates.

ENVIRONMENT

• HDX systems

PROCEDURE

1. The recommended filter is a disposable funnel with filter and fits standard media bottles. Below is a picture showing what it looks like.

   • Pour the buffers into the funnel, and then filter it out with a regular pump.

   • The filter from Corning is 0.22 um; part number 430626.

   • Disposable bottles are also available.

2. Filter all buffers with high concentration of Urea or Guanidine HCl.

   • For HDX, filter the pepsin wash and Buffer Q. Normally, you do not need to filter buffers E and L.

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ADDITIONAL INFORMATION