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HDX: How to filter solvents and buffers - WKB5268

Article number: 5268

OBJECTIVE or GOAL

Filtered solvents are less likely to clog up valves and pumps at low flow rates.

ENVIRONMENT

  • HDX systems

PROCEDURE

  1. The recommended filter is a disposable funnel with filter and fits standard media bottles. Below is a picture showing what it looks like.

  2. Filter all buffers with high concentration of Urea or Guanidine HCl.

    • For HDX, filter the pepsin wash and Buffer Q. Normally, you do not need to filter buffers E and L.

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ADDITIONAL INFORMATION

 

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