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Waters

Vion IMS QTof Fluidics Setup warning - WKB993

Article number: 993

SYMPTOMS

  • Fluidics setup warning in instrument pane and UNIFI logs
  • Acquisition stopped

ENVIRONMENT

  • Vion IMS QTof
  • UNIFI 1.8 and 1.8.1

CAUSE

  • Lockspray beam instability
  • Lockspray beam too intense or not intense enough
  • Poor mass accuracy

FIX or WORKAROUND

  1. Ensure that the lockspray beam is stable:
    1. Purge sample fluidics to remove trapped bubbles.
    2. Rebuild lockspray reference probe.
    3. Optimize reference probe capillary voltage.
  2. Ensure that the lockspray beam is within acceptable limits:
    1. Make up fresh Leucine Enkephalin lockspray solution at 50 pg/ul.
  3. Ensure that the lockspray beam is at an acceptable mass window before acquisition:
    1. Check the mass position of Leucine Enkephalin, and adjust if necessary.

ADDITIONAL INFORMATION

Fluidics set up checks that:

  • Beam is stable
  • Beam can be attenuated to 0.5 IPP and is stable
  • Mass accuracy of Leu Enk is within a certain window

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