How to link a calibration file to a tune page in MassLynx - WKB12335

OBJECTIVE or GOAL

Use a pre-existing calibration with a specific tune page.

Update the old tune page with the new calibration (e.g., post-PM).

ENVIRONMENT

• MassLynx
• SQD2
• Xevo TQ-MS
• Xevo TQD
• Xevo TQ-S
• Xevo TQ-XS
• Xevo TQ-S micro

PROCEDURE

1. In the tune page, select the Calibration drop-down menu > Linked Calibration.
2. Select Browse and browse to C:\MassLynx\Intellistart\Results\ (the desired resolution mode).
3. Select the most recent .cal file.
4. Adjust the tune page settings to have the same LM resolution, HM resolution, and ion energy as the settings in the calibration file.
5. The settings in the calibration file can be verified by going to Calibration drop-down menu > Calibration Instrument.
6. Do not make any changes in this page; just make note of the LM/HM/Ion Energy settings for MS1 and MS2, and then exit the window.
7. Make the suitable changes in the tune page. 

ADDITIONAL INFORMATION

Find the LM and HM resolution and ion energy settings that go with the calibration by doing the following:

• Once the calibration is linked, go to the tune page, select the calibration drop-down menu, and then select calibrate instrument. It will give the MS1 and MS2 LM and HM resolution settings and the ion energy (IE).
• The steps above work only for positive ion mode; for negative ion mode, browse to: C:\MassLynx\Intellistart.pro\ and open the Resolution_negative_Date.html to get the settings.