What process and acceptance criteria are associated with ACQUITY QDa/QDa II Mass Detectors for mass scale calibration and resolution? - WKB11532
ENVIRONMENT
- ACQUITY QDa Mass Detector
- ACQUITY QDa II Mass Detector
ANSWER
ACQUITY QDa/QDa II Mass Detectors Resolution Background
The ACQUITY QDa/QDa II Mass Detectors quadrupole resolution is set up to ensure that mass data is acquired across the mass range at a nominal resolution of 0.7 Da Full Width Half Maximum (FWHM). This means that mass spectral peaks across the range will be approximately 0.7 Da wide at half the maximum height of the peak (see tolerance described below). It is possible to both set up and check the quadrupole resolution of the ACQUITY QDa/QDa II Mass Detector. The resolution setup is performed in both positive and negative ion modes.
ACQUITY QDa/QDa II Mass Detectors Resolution Check Acceptance Criteria
For a successful resolution check, the following criteria must be met: The resolution check experiment must confirm that 4 out of 4 masses * (4 for positive/4 for negative) across the mass range have a resolution of 0.7 Da Full Width Half Maximum (FWHM) with an acceptance criteria of ±0.2 Da. Each peak must match the 0.7 Da peak width (within a tolerance of ±0.2) for both fast and slow scanning experiments. On completion of a successful resolution check, the expiration date shown in the ACQUITY Console will be updated; it will display the new expiration date of the resolution (setup date + 365 days).
ACQUITY QDa/QDa II Mass Detectors Resolution Setup Acceptance Criteria
For a successful resolution The resolution setup experiment must set 4 out of 4 masses * (4 for positive/4 for negative) across the mass range at a resolution of 0.7 Da Full Width Half Maximum (FWHM), with an acceptance criteria of ±0.2 Da. For both fast and slow scanning experiments; each peak must match the 0.7 Da peak width (with a tolerance of ±0.2).
* ACQUITY QDa II Mass Detector [Max end mass @ 1500 Da]
Negative Ion Peaks: 135, 467, 915 and 1413 Da.
Positive Ion Peaks: 169, 545, 877 and 1325 Da.
ACQUITY QDa Mass Detector [Max end mass @ 1250 Da]
Negative Ion Peaks: 135, 417, 749 and 1081 Da.
Positive ion peaks: 169, 479, 761 and 1159.
On completion of a successful resolution setup, the ACQUITY Console displays the new expiration date of the resolution (setup date 365 days). If unsuccessful, the resolution expiration date will be blank. After a completed resolution setup, a resolution check is performed to verify its success (see below). A re-run of the resolution setup instigates the requirement to perform the QDa mass scale calibration setup.
ACQUITY QDa/QDa II Mass Detectors Mass Scale Calibration Background
On all Waters single quadrupole and tandem quadrupole MS instruments the mass scale calibration setup is performed in a single ionization mode and applied to both positive and negative modes. Because this process calibrates the voltages applied to quadrupoles, it is independent of the polarity used. A successful calibration setup can be applied to each ionization mode. On the ACQUITY QDa/QDa II Mass Detectors, the mass scale calibration is performed in negative ion mode. It is possible to both set up and check the mass scale calibration of the ACQUITY QDa/QDa II Mass Detectors.
ACQUITY QDa/QDa II Mass Detectors Mass Scale Calibration Check Acceptance Criteria
For a successful calibration check, the following criteria must be met: 4 out of 6 ** masses must be within ±0.2 Da of the reference masses On completion of a successful calibration check, the ACQUITY Console displays the original calibration created date and the expiration date of the calibration remains unchanged (last recalibration date + 365 days). If unsuccessful, the calibration date shows as "Uncalibrated" and the expiration date is blank.
** ACQUITY QDa II Mass Detector (MinPeaksToPass: 4 out of 7 masses)
Negative Ion Peaks: 85, 135, 301, 467, 749, 1131 and 1413 Da.
ACQUITY QDa Mass Detector (MinPeaksToPass: 4 out of 6 masses)
Negative Ion Peaks: 85, 135, 301, 467, 749 and 1131 Da.
ACQUITY QDa/QDa II Mass Detectors Mass Scale Calibration Setup Acceptance Criteria
For a successful calibration setup, the following criteria must be met: 15 out 20 *** calibration peaks must be within ±0.2 Da of the reference mass. After a completed calibration setup, a calibration check is performed to verify its success. On completion of a successful calibration setup, the ACQUITY Console displays the new calibration created date (calibration date) and the new expiration date of the calibration (calibration date + 365 days). If unsuccessful, the calibration created date shows as "Uncalibrated".
*** ACQUITY QDa II Mass Detector (21 reference masses)
Negative Ion Peaks: 69, 85, 135, 185, 251, 301, 351, 417, 467, 517, 583, 749, 799, 849, 915, 965, 1081, 1131, 1181, 1247 and 1413 Da.
ACQUITY QDa Mass Detector (20 reference masses)
Negative Ion Peaks: 69, 85, 135, 185, 251, 301, 351, 417, 467, 517, 583, 749, 799, 849, 915, 965, 1081, 1131, 1181, 1247Da.
ADDITIONAL INFORMATION