OBJECTIVE or GOAL
Clean the sample cone and cone gas nozzle when the following conditions apply:
- The sample cone and cone gas nozzle are visibly fouled/dirty.
- You have dismissed LC and sample-related causes for decreased signal intensity.
- Consider cleaning the sample cone and cone gas nozzle on a routine basis depending on MS usage.
Follow the maintenance procedures in the relevant MS Operator's Overview and Maintenance Guide for safety precautions and materials required.
- All Waters ESI (electrospray) Mass Spectrometers
- Remove the sample cone assembly following the procedure in the relevant MS Operator's Overview and Maintenance Guide.
- Remove the o-ring (and the peek handle if present) from the sample cone.
- Place the cone and sample cone nozzle on their flat base in a clean glass beaker/vessel (never on the tip as it can damage the cone) containing 1:1 methanol:water.
- If the components are visibly contaminated, use 45:45:10 methanol/water/formic acid.
- Place the beaker/vessel in an ultrasonic bath for 30 minutes.
- If using formic acid in the cleaning solution, rinse with water and place in a glass beaker with 100% water.
- Place the beaker/vessel in an ultrasonic bath for 20 minutes.
- Remove any residual water from components by putting them in a glass beaker/vessel with 100% methanol.
- Place the beaker/vessel in an ultrasonic bath for 10 minutes.
- Carefully remove the components from the glass beaker and blow dry them with inert, oil-free gas.