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What is the best flow cell for an ACQUITY PDA running large molecules/proteins that exhibit peak tailing? - WKB9536

Article number: 9536

ENVIRONMENT

  • ACQUITY H-Class System ACQUITY PDA Detector Protein/amino acid analysis.

ANSWER

In some applications the ACQUITY Light Guided flow cells exhibit significant peak tailing due to adherence of the large molecules to the Teflon AF.  Eliminating the Teflon AF and going to a design with a stainless steel body is an acceptable solution. The 5-mm stainless steel PDA flow cell 205000612) is about half as sensitive as the ACQUITY LG PDA flow cell because the path length is reduced from 10 mm to 5 mm. There is also an increase in volume, which slightly increases the dispersion of this flow cell.

ADDITIONAL INFORMATION

ACQUITY PDA 5mm SS FC [205000612].

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