How is the carryover test calculated for the SystemsQT project for the Alliance 2695? - WKB91577

ENVIRONMENT

• Alliance HPLC System
• 2695 Separations Module
• Waters SystemsQT

ANSWER

The injector carryover test determines the presence of sample carryover from one injection to the next. Two (2) pre-blank injections are performed to determine whether the system is clean enough to measure injector carryover. These injections are followed by three (3) caffeine calibration injections at a concentration of 0.4 μg/mL with an injection volume of 10.0 μL, which corresponds to 0.01% of the challenge injection. The challenge injection is made at a concentration of 4.0 mg/mL with an injection volume of 10.0 μL, followed by three (3) post-blank injections. The test passes if the % injector carryover is
< or = 0.01%, or the peak is missing for PostBlank1.

ADDITIONAL INFORMATION