First injection with ACQUITY FTN is inaccurate - WKB89100

SYMPTOMS

• First injection area count is lower than subsequent injections

ENVIRONMENT

• ACQUITY Arc
• ACQUITY Arc Sample Manager FTN-R
• ACQUITY UPLC I-Class
• ACQUITY UPLC H-Class PLUS
• ACQUITY UPLC H-Class PLUS Bio
• ACQUITY UPLC I-Class PLUS
• ACQUITY UPLC H-Class
• ACQUITY UPLC H-Class Bio
• ACQUITY UPLC Sample Manager - Flow Through Needle PLUS (SM-FTN PLUS)

CAUSE

The septa on vials are not pre-slit.

FIX or WORKAROUND

1. Determine if a clog is present in the needle (How to test your FTN needle for a partial clog).
2. Consider use of pre-slit septa.

ADDITIONAL INFORMATION