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Error: Failed to Calibrate System Volume on ACQUITY Sample Manager Fixed Loop - WKB8630

Article number: 8630

SYMPTOMS

  • Failed to calibrate system volume
  • All other diagnostics pass (Syringe Leak, BSM Dynamic Leak, VDD calibrates reproducibly, and so on)

ENVIRONMENT

  • M-Class Sample Manager Fixed Loop (SM-FL)
  • ACQUITY UPLC Sample Manager
  • ACQUITY I-Class SM-FL
  • Solvent 100% Methanol or 100% organic solvent

CAUSE

  • VDD is not recognizing the air gap that it is seeking during the pressurized portion of the characterization.
    • Observed rise in pressure during the pressurized measurement, then the failure will occur (VDD doesn't find the air gap)
    • The characterization will stop and the pressure will slowly drift lower.

FIX or WORKAROUND

  1. Switch to appropriate solvent or organic and water
  2. Increase the size of the air gaps:
    • Edit the instrument/inlet method where custom air gaps are programmed between 6uL and 8uL (the automatic value is 4 uL).
    • Setup or load the method in order for the instrument to accept the new set air gap values.
    •  Needle Loop and Characterization will use the conditions of the last valid (setup/loaded) method to perform the characterization.
      • The larger air gaps prevent the organic solvent from completely dissolving the air gaps.

ADDITIONAL INFORMATION

Failing Test Pressure Trace:


Passing Test Pressure Trace

 

Also see Error: Failed to Calibrate System Volume on FL ACQUITY M-Class - Solution #2

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