HPLC chromatogram has a lower peak area value than before - WKB86274

SYMPTOMS

• Measurement was performed using 2695/2489/2475. Only the chromatogram from 2489 had a smaller peak area value than before (the peak area value from 2475 was the same as before)
• The peak area value from 2489 is about 1/3-1/4 of the previous
• Two components are measured, both areas are low at a same level
• Retention time and peak shape are the same as before
• 2489 lamp hours is 1000 hours

ENVIRONMENT

• 2487 Dual-Wavelength Absorbance Detector
• 2489 UV/Vis Detector
• 2475 Multi-Wavelength Fluorescence Detector
• 2695 Alliance Separations Module

CAUSE

• Poor sensitivity
• Dirty system

FIX or WORKAROUND

1. Perform the system cleaning
2. Perform the manual calibration
3. After equilibration with the initial composition of the measurement conditions, restart the detector

ADDITIONAL INFORMATION

Routine Cleaning for the Alliance System