How to install an extension loop on ACQUITY H-Class FTN? - WKB8352

OBJECTIVE or GOAL

How to install an extension loop on ACQUITY H-Class FTN.

ENVIRONMENT

• ACQUITY H-Class FTN (flow-through needle), Sample Manager 
• ACQUITY UPLC I-Class PLUS

PROCEDURE

To install or replace an extension loop: 

1. Power-off the sample manager.
2. Open the fluidics compartment door.
3. Using the 1/4-inch open-end wrench, unscrew the fitting of the needle tubing or extension loop from port of the injection valve.
   
    
   Needle tubing (or extension loop tubing, if installed)
   
    
4. Fully insert the extension-loop tubing into port 4 of the injection valve, and then thread the fitting into the port.
5. Holding the extension loop tubing against the bottom of the port, finger-tighten the extension-loop fitting.
6. Using the 1/4-inch open-end wrench, tighten the extension-loop fitting an additional 1/6-turn (for existing fittings) or 3/4-turn (for a new fitting).
   
   Note: Failure to properly bottom the extension loop tubing in the port can result in carryover or poor chromatography.
    
7. Screw the sample needle's fitting into the extension-loop union, and then use the 1/4-inch open-end wrench to tighten the fitting 3/4-turn beyond finger-tight (for a new fitting) or 1/6-turn beyond finger-tight (for existing fittings).
   
   Note: Failure to properly bottom the sample needle tubing in the extension-loop union could result in carryover or poor chromatography.
   
   
     Extension loop Union      Extension loop tubing   Extension loop fitting
   
    
8. Close the fluidics compartment door.
9. Power-on the sample manager.
10. Modify the volume of the extension loop according to the following instructions.

To modify the needle or extension-loop volume setting:

1. In the console, select the sample manager, and then click Configure > Volumes.
2. In the Volume Configuration dialog box, select the appropriately sized needle or extension loop size from the list, and then click OK.
   
   Note: If the sample manager is not fitted with an extension loop, select 0 as the loop size

ADDITIONAL INFORMATION

For part numbers on extension loops, see WKB6746

Flow Through Needle Overview and Maintenance guide