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High system pressure on ACQUITY UPLC Sample Manager - Flow Through Needle - WKB81819

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Article number: 81819

hSYMPTOMS

  • High pressure
  • System overpressure error
  • Issue observed when in inject position, not in load position

ENVIRONMENT

  • ACQUITY Arc
  • ACQUITY Arc Bio
  • ACQUITY Arc FTN-R
  • ACQUITY Arc Bio FTN-R
  • ACQUITY Arc Premier FTN-R
  • ACQUITY UPLC Sample Manager - FTN
  • ACQUITY UPLC I-Class FTN

CAUSE

Blockage, clog, or bend of needle and/or needle seat

FIX or WORKAROUND

  1. Replace the needle and needle seat.
  2. Calibrate needle z axis

Part numbers for replacement needles (these parts include the needle seat):

700005279 - 30-µL stainless steel needle

700009578 - 30-µL MP35N needle (Bio)

700005215 - 15-µL stainless steel needle

700005421 - 15-µL MP35N needle (Bio)

700008977 - Sample Needle Kit, 15 µL, SS

700011628 - Sample Needle Kit, 15 µL Textured

700005279 - Sample Needle Kit, 30 µL, SS

700011698 - Sample Needle Kit, 30 µL, SS, Textured (PLUS)

700012822 - Sample Needle Kit, 30 µL, HPS

ADDITIONAL INFORMATION

These issues are often caused by use of applications that employ relatively high concentrations of non-volatile buffer salts in the mobile phases—typically applications taken from pharmacopoeias (USP/Ph.Eur./JP, others).

When using such procedures, it is recommended that the system be flushed and cleaned regularly to remove excess buildup of buffer salts, which can result in pressure issues at various locations within the system.

id81819, eluent, UPAQSM, UPARBQSM, UPARBSMFTN, UPARBSMFTN, UPARCH30, UPARHQSM, UPARHSMFTN, UPARPFTN, UPARQSM, UPARSMFTN, UPARSMFTN, UPIBSM, UPIBSM+, UPISMFL, UPISMFL+, UPISMFTIVD, UPISMFTN, UPISMFTN+, UPPDAARCB, UPQSM, UPQSM+, UPQSMBIO, UPQSMBIO+, UPSMFTN, UPSMFTN+, UPSMFTNB+, UPSMFTNBIO

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