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Retention times shift out in ACQUITY UPLC H-Class - WKB76868

Article number: 76868

SYMPTOMS

  • Pressure is stable
  • Pressure is lower than normal
  • Retention times shift out

ENVIRONMENT

  • ACQUITY UPLC H-Class
  • ACQUITY UPLC H-Class Bio
  • ACQUITY UPLC I-Class
  • ACQUITY UPLC H-Class PLUS
  • ACQUITY UPLC H-Class PLUS Bio
  • ACQUITY UPLC I-Class PLUS
  • ACQUITY Arc
  • ACQUITY APC Sample Manager - FTN
  • ACQUITY UPLC Sample Manager - Flow Through Needle PLUS (SM-FTN PLUS)

CAUSE

Leaking needle seal (405011492)

FIX or WORKAROUND

  1. Replace the needle seal.
  2. Purge the fluidics.
  3. Perform needle seal characterization.
  4. Perform a dynamic leak test on the injector pod.

ADDITIONAL INFORMATION

Consider replacing the needle as well. A blunt tip can cause premature seal failure.

The part number for the standard 15-µL needle with vespel seat is 700005215.

Video for replacement of needle and vespel seat (needle seal)

Needle replacement written procedure

See pages 68-83 of the FTN Overview and Maintenance Guide for the needle seal replacement procedure.

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