Retention times shift out in ACQUITY UPLC H-Class - WKB76868
Article number: 76868
SYMPTOMS
- Pressure is stable
- Pressure is lower than normal
- Retention time shifts during sample set
- The dynamic leak end point vent valve test passes
- The dynamic leak test with column end point passes
ENVIRONMENT
- ACQUITY UPLC H-Class
- ACQUITY UPLC H-Class Bio
- ACQUITY UPLC I-Class
- ACQUITY UPLC H-Class PLUS
- ACQUITY UPLC H-Class PLUS Bio
- ACQUITY UPLC I-Class PLUS
- ACQUITY Arc
- ACQUITY APC Sample Manager - FTN
- ACQUITY UPLC Sample Manager - Flow Through Needle PLUS (SM-FTN PLUS)
CAUSE
Leaking needle seal (405011492)
FIX or WORKAROUND
- Replace the needle seal.
- Purge the fluidics.
- Perform needle seal characterization.
- Perform a dynamic leak test on the injector pod.
ADDITIONAL INFORMATION
Consider replacing the needle as well. A blunt tip can cause premature seal failure.
The part number for the standard 15-µL needle with vespel seat is 700005215.
Video for replacement of needle and vespel seat (needle seal)
Needle replacement written procedure
id76868, A-30SM, DLT, UPAQSM, UPARBQSM, UPARBSMFTN, UPARCH30, UPARHQSM, UPARHSMFTN, UPARQSM, UPARSMFTN, UPBSMIVD, UPIBSM, UPIBSM+, UPIBSMIVD, UPISMFL, UPISMFL+, UPISMFTIVD, UPISMFTN, UPISMFTN, UPISMFTN+, UPLCCOLHTR, UPNBINARY, UPPDAARCB, UPPFTN, UPQSM, UPQSM+, UPQSMBIO, UPQSMBIO+, UPSMFTN, UPSMFTN, UPSMFTN+, UPSMFTN+, UPSMFTNB+, UPSMFTNBIO, UPSMIVD, UPSMPMGR