What are the injection volume recommendations for the ACQUITY UPLC Sample Manager-FTN? - WKB67734

ENVIRONMENT

ACQUITY UPLC H-Class Sample Manager- Flow Through Needle (SM-FTN)
ACQUITY UPLC I-Class SM-FTN
ACQUITY Arc Premier
ACQUITY Premier FTN

ANSWER

Configuration	Extension loop	Injection volume
15-µL needle	-	0.1 to 10 µL
30-µL needle	-	0.1 to 25 µL
Any needle	50 µL	Up to 50 µL
Any needle	100 µL	Up to 100 µL
Any needle	250 µL	Up to 250 µL

Accuracy (aspiration) ±0.2 µL, measured by fluid weight removed from vial with 10.0 µL injections averaged over 20 injections using standard 100 µL syringe
Linearity >0.999 (standard needle) caffeine 0.030 mg/mL, ACN/H2O (10:90), isocratic 0.6 mL/min, 0.2 to 10.0 µL, 1 to 70% needle volume
Precision <1% RSD 0.2 to 1.9 µL <0.5% RSD 2.0 to 10.0 µL

ADDITIONAL INFORMATION

The maximum injection volume is 75% of the total loop and needle volume. Injections larger than the recommended maximum volume can result in reduced sample recovery.

Apply the following formula to calculate the maximum injection volume:

Maximum injection volume (μL) = [loop volume (μL) + needle volume (μL)] × 0.75

Example: For a system fitted with a 250-μL loop and 30-μL needle, the maximum injection volume is 210 μL.

Maximum injection volume = [250 μL + 30 μL] × 0.75
Maximum injection volume = 280 μL × 0.75
Maximum injection volume = 210 μL

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