What are the injection volume recommendations for the ACQUITY UPLC Sample Manager-FTN? - WKB67734
Article number: 67734
ENVIRONMENT
- ACQUITY UPLC H-Class Sample Manager- Flow Through Needle (SM-FTN)
- ACQUITY UPLC I-Class SM-FTN
- ACQUITY Arc Premier
- ACQUITY Premier FTN
ANSWER
| Configuration | Extension loop | Injection volume |
| 15-µL needle | - | 0.1 to 10 µL |
| 30-µL needle | - | 0.1 to 25 µL |
| Any needle | 50 µL | Up to 50 µL |
| Any needle | 100 µL | Up to 100 µL |
| Any needle | 250 µL | Up to 250 µL |
- Accuracy (aspiration) ±0.2 µL, measured by fluid weight removed from vial with 10.0 µL injections averaged over 20 injections using standard 100 µL syringe
- Linearity >0.999 (standard needle) caffeine 0.030 mg/mL, ACN/H2O (10:90), isocratic 0.6 mL/min, 0.2 to 10.0 µL, 1 to 70% needle volume
- Precision <1% RSD 0.2 to 1.9 µL <0.5% RSD 2.0 to 10.0 µL
ADDITIONAL INFORMATION
The maximum injection volume is 75% of the total loop and needle volume. Injections larger than the recommended maximum volume can result in reduced sample recovery.
Apply the following formula to calculate the maximum injection volume:
Maximum injection volume (μL) = [loop volume (μL) + needle volume (μL)] × 0.75
Example: For a system fitted with a 250-μL loop and 30-μL needle, the maximum injection volume is 210 μL.
- Maximum injection volume = [250 μL + 30 μL] × 0.75
- Maximum injection volume = 280 μL × 0.75
- Maximum injection volume = 210 μL
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