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Waters

Negative peaks observed using Refractive Index Detector - WKB67342

Article number: 67342

SYMPTOMS

  • Negative peaks observed using Refractive Index Detector
  • Negative peaks have the same peak shape and intensity (but mirrored) as the positive peaks measure in the chromatogram

ENVIRONMENT

  • 2410 Differential Refractometer
  • 2414 Refractive Index Detector
  • ACQUITY RI Detector

CAUSE

Pressure relief valve activated

FIX or WORKAROUND

Reduce back pressure behind the RI detector (waste line blocked or clogged)

ADDITIONAL INFORMATION

During normal operation, the pressure relief valve remains closed. It opens when the pressure for the flow cell, whose maximum pressure rating is 690 kPa (6.89 bar, 100 psi) gets too high. This protects the flow cell from exceeding the maximum pressure rating. The pressure rating of the relief valve is 103.4 kPa (1 bar, 15 psi).

The attachments show the flow path when pressure relief valve is open or closed. When closed (normal operation), sample passes through the sample side of the flow cell only (positive peaks). When open, sample first passes through the sample side of the flow cell (positive peaks), and later through the reference side of the flow cell (generating the mirrored signal observed before for the positive peaks).

 

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