Loss of prime on Alliance 2695 when running a gradient method - WKB6212

SYMPTOMS

• Loss of prime

ENVIRONMENT

• Alliance HPLC System
• 2695 Separations Module
• 2695D Dissolution Separations Module
• 2795 Separations Module

CAUSE

Running above 2 mL/minute

FIX or WORKAROUND

1. Replace the GPV tubing with the High-flow GPV inlet tubing (WAT270968) when running at 2 mL/min or higher.
    

ADDITIONAL INFORMATION

WAT270986  2690 GPV - PRIMARY HD TUBE  (.030 SS) Standard Flow SS tubing in black Teflon tubing
WAT270976  2690 LO FLOW IN TUBE (.020 SS)
WAT270968  2690 HIGH FLOW INLET TUBING   (.040 SS)