ACQUITY UPLC Sample Manager error "Failed to Calibrate system volume" - WKB60416

SYMPTOMS

• Error "Failed to Calibrate system volume" when characterizing the needle and loop
• Error "Failed to Calibrate system volume" when calibrating the VDD
• Error "Sample Low pressure"

ENVIRONMENT

• ACQUITY UPLC Sample Manager 

CAUSE

Incorrect sample loop and/or sample syringe installed

FIX or WORKAROUND

1. Install the correct sample syringe and/or sample loop.
2. Make sure to change the sample syringe and/or sample loop under Configure>volumes.
3. Recalibrate VDD.
4. Characterize needle seal.
5. Characterize the needle and loop volumes.

ADDITIONAL INFORMATION