ACQUITY H-Class SM-FTN Maintain Test Procedures - WKB5971
OBJECTIVE or GOAL
Confirm Sample Manager (SM-FTN) status.
ENVIRONMENT
- ACQUITY UPLC H-Class Sample Manager with Flow-Through Needle
- ACQUITY UPLC I-Class Sample Manager with Flow-Through Needle
- ACQUITY UPLC Premier Sample Manager with Flow-Through Needle
- ACQUITY UPLC Arc Sample Manager with Flow-Through Needle - With installed transducer
- MassLynx
- Empower
- Waters Connect -See additional information
PROCEDURE
- Characterize Needle Seal:
In the console, go to SM-FTN > Maintain > Characterize needle seal and click Start.
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If necessary, perform a Z-axis calibration before running the test, as it ensures proper alignment of the needle.
During the test, the needle will drop into the injection port and push against the spring to measure the seal constant. Ensure all components, such as the spring and needle seat, are properly aligned and functional.
After completing the test, verify that the seal constant falls within the acceptable range (8-11 N/mm). If the value is outside this range, inspect and adjust the needle seal components or replace them if necessary.
2. Leak test - Sample Syringe:
In the console, go to SM-FTN > Maintain > Leak test and select Start.
The sample syringe leak test verifies the sample syringe, metering syringe, valve, sample transducer and inject valve are free of leaks. This test also verifies the sample syringe is properly primed and no bubbles are present.
3. Needle Seal Readiness Test:
Establish an appropriate pump flow and pressure ((e.g., ~0.6 mL/min for H/I-Class systems) to obtain a column backpressure of 6000-8000 psi.)
In the console, go to SM-FTN > Maintain > Needle seal readiness test and select Start.
- 4. System leak test
In the console, go to QSM > Maintain > Leak Test.
- Select an appropriate channel (A, B, C or D) with organic solvent. For BSM select A or B.
As end point select column, include Sample Manager Needle and Seal.
Install a pin plug in port 6 of the Sample Manager POD Injector. (What is the part number for the v-detail pin plug for the Acquity Systems? - WKB8137)
Click Start.
ADDITIONAL INFORMATION
Acquity UPLC Sample Manager - Flow Through Needle Overview and Maintenance guide
How to Resolve Sample Manager FTN Leak Tests Failures? - wkb341745
Waters Connect:
- How to perform needle seal readiness test diagnostic in Waters_Connect? - wkb319814
How to perform a leak test at the sample manager in Waters connect - WKB320899
How to perform a Dynamic Leak test in Waters connect - WKB319812