ACQUITY UPLC H-Class runs at a lower pressure than normal - WKB56050

SYMPTOMS

• Typical run pressure is 2,000–4,000 psi lower than normal
• System fails dynamic leak test only when the needle and seat are included
• Needle seal readiness test fails

ENVIRONMENT

• ACQUITY UPLC H-Class
• ACQUITY UPLC H-Class Bio
• ACQUITY PREMIER
• ACQUITY UPLC Sample Manager - Flow Through Needle PLUS (SM-FTN PLUS)

CAUSE

The needle seat has worn and no longer makes a good seal.

FIX or WORKAROUND

1. Replace the needle and needle seat.
2. Run z axis calibration, needle seal characterization, and the needle seal readiness check after replacement.

ADDITIONAL INFORMATION

Sample needle part numbers

• 15uL H-Class: 700005215
• 15uL H-Class Bio: 700005421
• 15uL H-Class Plus (Textured): 700011628
• 15uL H-Class Plus Bio (Textured): 700011658
• 15uL ACQUITY Premier HPS (Textured): 700012820
• 15uL ACQUITY Premier HPS: 700012821

Vespel seat Part number: 405011492

Seat port assembley part numbers:

• Seat Port Assembly, .004 ID: 700005234
• Seat Port Assembly, .004 ID, Bio: 700005682
• Seat Port Assembly, HPS: 700012823