How to adjust the O-ring in an I-Class or ACQUITY UPC2 SM-FL - WKB319503

OBJECTIVE or GOAL

Adjust the Sample Manager - Fixed Loop (SM-FL) O-ring.

ENVIRONMENT

• ACQUITY UPLC I-Class
• ACQUITY UPLC I-Class PLUS
• ACQUITY UPC² System

PROCEDURE

• Open the sample compartment door and the fluidics compartment door.
• Using a T20 TORX driver, loosen the two captive screws that secure the access panel.

• Insert a piece of used PEEK needle or PEEK tubing into the needle wash seal port, and gently push the needle down through the O-ring. You should feel slight resistance as the needle slides through the O-ring.

• If you do not feel slight resistance while sliding the needle through the O-ring, the needle wash seal port is probably too tight or too loose. Manually adjust the needle wash seal port until you can verify that it is seated correctly. (CAUTION: TO AVOID DAMAGING THE O-RING SEAL AND NEEDLE, DO NOT OVER-TIGHTEN THE NEEDLE WASH SEAL PORT).
•  Remove the PEEK needle or PEEK tubing from the needle wash seal port.

ADDITIONAL INFORMATION

Video Transcript