Skip to main content
Waters

No baseline with 2475 Fluorescence detector - WKB3192

Article number: 3192

SYMPTOMS

  • Low lamp hours
  • Failed to Normalize, gives error peak location not near 397 nm. Is there water in cell? Values not saved (40100).

ENVIRONMENT

  • ACQUITY FLR
  • 2475 detector (Fluorescence detector)

CAUSE

Detector saturated.

FIX or WORKAROUND

  1. Run fresh HPLC-grade methanol through flowcell for 15 minutes.
  2. Run fresh HPLC water through flowcell for 15 minutes.
  3. Reboot the detector.
  4. Perform manual wavelength calibration (Shift+3, also see manual).
  5. Perform normalization per 2475 manual. If you have an ACQUITY FLR, see the following screen shot:

Link to 2475 operator's guide / manual

Link to ACQUITY FLR guide

ADDITIONAL INFORMATION

 


Not able to find a solution? Click here to request help.