ACQUITY UPLC Sample Manager Needle Error: "Sample Fluidics High Pressure Limit" Error - WKB27524

SYMPTOMS

• Instrument produces "Sample Fluidics High Pressure Limit" Error during injection cycle
• Instrument produces "Sample Fluidics High Pressure Limit" Error during priming of wash solvents

ENVIRONMENT

• ACQUITY UPLC
• ACQUITY UPLC I-Class
• nanoACQUITY UPLC
• ACQUITY Sample Manager
• ACQUITY M Class

CAUSE

Clog inside sample needle or bent sample needle

FIX or WORKAROUND

1. Replace sample needle.
2. Prime wash solvents and sample syringe for at least three cycles.
3. Calibrate X-, Y- and Zp positions.
4. Calibrate needle z axis position.
5. Calibrate needle seal.
6. Calibrate needle and loop volumes.
7. Perform sample syringe and wash syringes leak tests.
8. Test the system with known standard.

ADDITIONAL INFORMATION

nanoACQUITY needle p/n 700002708

Standard ACQUITY PEEK needle p/n 700002644

I Class & M Class Needle 700005926