How to flush an ACQUITY Premier - WKB269769

OBJECTIVE or GOAL

Flush or clean the ACQUITY Premier System within the limits of High Performance Surfaces. (Order the LC Purging Kit (186010210) to perform this procedure.

ENVIRONMENT

• ACQUITY Premier

PROCEDURE

1. Remove the column and put a union in its place.
2. Plumb the outlet of the union to waste, not the MS (optionally, use a back-pressure line).
3. BASE Flush: Place all lines in a 0.1% aqueous ammonia solution in water.
4. SM: Prime all lines for 5 minutes each. FTN: 60s Wash, 30 cycles on syringe. -or- FL: 15 cycles
5. Run the BASE solution through the lines at 0.2 mL/min for 20 min (this can be extended if needed).
6. Place all lines in fresh, 100% water (LC-MS grade/MilliQ).
7. SM: Prime all lines for 5 minutes each. FTN: 60s Wash, 30 cycles on syringe. -or- FL: 15 cycles
8. Run the water through the system at 0.2 mL/min until the pressure is stable.
9. Place all lines in the IPA Flush: 98% IPA /2% water
10. SM: Prime all lines for 5 minutes each. FTN: 60s Wash, 30 cycles on syringe. -or- FL: 15 cycles
11. Run the IPA solution through the system at 0.1 mL/min for 20 min (this can be extended).
12. Optional: If the column is compatible with the ACID Flush, connect the column so that it can also be flushed.
13. ACID Flush: Place all lines in ACN:IPA:MeOH:H2O and 0.2% formic acid.
14. SM: Prime all lines for 5 minutes each. FTN: 60s Wash, 30 cycles on syringe. -or- FL: 15 cycles
15. Run the ACID solution through the system at 0.2 mL/min for 20 min (this can be extended).
16. Make up fresh mobile phase and connect the column to the MS.
17. SM: Prime all lines for 5 minutes each. FTN: 60s Wash, 30 cycles on syringe. -or- FL: 15 cycles
18. Run

ADDITIONAL INFORMATION