Baseline noise in 2414 - WKB26125
Article number: 26125
SYMPTOMS
- High baseline noise in 2414
- Sample and reference flow cell thoroughly purged
- Temperature set on the column and on the detector
- Very low pump pressure ripple
ENVIRONMENT
- ACQUITY UPLC RI Detector
- Alliance HPLC System
- 2695 Separations Module
- 2414 Refractive Index Detector
CAUSE
The mobile phase is mixed by the 2695 pump, generating slight turbulence that you can see in the 2414 chromatogram baseline.
FIX or WORKAROUND
Pre-mix the mobile phase in a bottle.
ADDITIONAL INFORMATION
id26125, 2414, 2414B, 2690, 2690D, 2690DUPGD, 2690UP, 2695, 2695D, 2695DE, 2695DEB, 2695DUP, 2695E, 2695EB, 2695INERT, 2695UP, A-10RI, A-10SSM, baseline ripple, eluent, pressure fluctuation