Degasser vacuum hardware errors on ACQUITY UPLC BSM solvent manager - WKB193648

SYMPTOMS

• Degasser vacuum hardware errors on ACQUITY UPLC BSM solvent manager

ENVIRONMENT

• ACQUITY UPLC BSM

CAUSE

The degasser chambers were insufficiently primed with fresh solvent

FIX or WORKAROUND

1. Prime each line in 100% methanol for five minutes.
   • Caution: Flush with a high aqueous intermediate solvent before moving to 100% methanol if the previous solvents are unknown or contained buffers.
2. If no further errors are observed, switch to an appropriate intermediate solvent and prime only the lines that will be used before returning to the required mobile phase.

ADDITIONAL INFORMATION