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Waters

What is the temperature band on ACQUITY column managers and autosamplers? - WKB14255

Article number: 14255

ENVIRONMENT

  • Empower 2
  • Empower 3
  • MassLynx
  • Column manager (CM)
  • ACQUITY sample managers (SM-FTN and FL)

ANSWER

The sample compartment starts the injection as soon as the temperature reaches the band, and it does not check the temperature of the chamber during the run.

The column manager checks if the column temperature is within plus or minus one-half of the value of the alarm band. For example: If you enter 40 ºC for the column temperature and ± 2.0 ºC for the alarm band, injections proceed if the sample is between 39 and 41 ºC. The reason for this is to avoid column temperature alarms during the injection (the column temperature is monitored during the run).

After the injection sequence starts and during the run, the full specified band is used (for example, if the set point is 40 ºC and the band is +/- 2.0 ºC, the injection sequence is not interrupted if the column temperature is between 38 and 42 ºC).

ADDITIONAL INFORMATION

For further information, see the help guide under the section "Setting column temperature".

"First, you enter the temperature set point to which the column compartment heats or cools. Then, if necessary, you can enter an alarm band that defines the temperature range within which the column manager allows sample injection. The software verifies that the temperature at the start of an injection is within the range defined by the set point, plus or minus one-half of the value of the alarm band.

Example: If you enter 25 ºC for the column temperature and ± 5.0 ºC for the alarm band, injections proceed if the sample is between 22.5 and 27.5 ºC. (One-half of the alarm band is 2.5.)

If the temperature is outside the set range, "Waiting for Column Temperature" appears while the column manager cools or heats to within the temperature range. If it fails to reach this range, "Column Temperature Range Error" appears."

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