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Waters

Retention time shift and poor chromatographic resolution of peaks after the first injections - WKB12700

Article number: 12700

SYMPTOMS

  • First injections, peaks elute at correct retention times
  • Second injection, peaks elute at much earlier time and nearly co-elute
  • Third injection, peaks co-elute at very early retention time
  • Found: eluant is more than 30 days old

ENVIRONMENT

  • ACQUITY UPLC H-Class
  • HILIC Column
  • Reverse-Reverse Phase chromatography
  • Eluant: Acetonitrile (no additives), Water (0.05 mMol Ammonium Acetate)

CAUSE

Acqueous solvent degraded

FIX or WORKAROUND

Prepare new solvent

ADDITIONAL INFORMATION

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