Alliance 2695 Separations Module loses prime or cannot prime - WKB12448
Article number: 12448
SYMPTOMS
- Prime lost during gradient run using A and B solvent lines; able to run same gradient with C and D solvent lines
- New pump seals and check valves
- New tubing from the GPV to the primary check valve
- New GPV
- Degasser pressure okay
- Solvent line has flow at chamber inlet
- Minimal or no flow out of degasser chamber to the GPV
- Solvent filters and solvent lines already replaced
- Degasser shows 0.4 psi
ENVIRONMENT
- Alliance HPLC System
- 2695 Separations Module
- 2695D Dissolution Separations Module
- 2795 Separations Module
CAUSE
Problem with degasser chambers for solvent lines in question
FIX or WORKAROUND
Replace the degasser chambers—Performance Plus Degasser Chamber (700011709).
- Turn the instrument OFF and disconnect the external AC power source.
- Remove the solvent lines from the solvent bottles and drain the solvent from the tubes.
- Remove the vent tube from the exhaust vent.
- Open the lower compartment doors.
- Loosen the captive screw that secures the solvent conditioning tray to the chassis.
- While holding the inlet check valve stationary using a 1/2-inch wrench, disconnect the stainless steel tube from the primary head check valve.
- Remove the right- and left-hand side panels from the instrument.
- Disconnect the GPV cable from the pump driver board (the cable runs through cable ties secured to the bottom of the sample compartment).
- Remove the GPV solvent inlet and GPV lines from the degasser chambers.
- Unclip the plastic solvent line brackets and remove all associated tubing (needle wash, injector waste).
- Remove the solvent conditioning tray from the instrument.
- Remove the vacuum manifold tubing from the degasser chambers.
- Install the new degasser chambers, and then follow these steps in reverse order, being sure to correctly reconnect the GPV lines and cables.
ADDITIONAL INFORMATION