Alliance 2695 Separations Module loses prime or cannot prime - WKB12448

SYMPTOMS

• Prime lost during gradient run using A and B solvent lines; able to run same gradient with C and D solvent lines 
• New pump seals and check valves
• New tubing from the GPV to the primary check valve
• New GPV
• Degasser pressure okay
• Solvent line has flow at chamber inlet
• Minimal or no flow out of degasser chamber to the GPV
• Solvent filters and solvent lines already replaced
• Degasser shows 0.4 psi

ENVIRONMENT

• 2695 Separations Module

CAUSE

Problem with degasser chambers for solvent lines in question

FIX or WORKAROUND

Replace the degasser chambers—Performance Plus Degasser Chamber (700011709).

1. Turn the instrument OFF and disconnect the external AC power source.
2. Remove the solvent lines from the solvent bottles and drain the solvent from the tubes.
3. Remove the vent tube from the exhaust vent.
4. Open the lower compartment doors.
5. Loosen the captive screw that secures the solvent conditioning tray to the chassis.
6. While holding the inlet check valve stationary using a 1/2-inch wrench, disconnect the stainless steel tube from the primary head check valve.
7. Remove the right- and left-hand side panels from the instrument.
8. Disconnect the GPV cable from the pump driver board (the cable runs through cable ties secured to the bottom of the sample compartment).
9. Remove the GPV solvent inlet and GPV lines from the degasser chambers.
10. Unclip the plastic solvent line brackets and remove all associated tubing (needle wash, injector waste).
11. Remove the solvent conditioning tray from the instrument.
12. Remove the vacuum manifold tubing from the degasser chambers.
13. Install the new degasser chambers, and then follow these steps in reverse order, being sure to correctly reconnect the GPV lines and cables.

ADDITIONAL INFORMATION