Low sensitivity with PDA - WKB123038

SYMPTOMS

• Low sensitivity on LC-UV system
• Read energy - 25,000 at 230 with water in flow cell
• Injections look like blanks (500 µL in 2-mL vial)
• Peak is present when injecting 2 mL in 2-mL vial

ENVIRONMENT

• ACQUITY UPLC H-Class Sample Manager - FTN
• ACQUITY UPLC PDA Detector

CAUSE

Sample Manager requires z-axis calibration.

FIX or WORKAROUND

Calibrate Z-Axis per WKB28509.

ADDITIONAL INFORMATION