How to plumb and configure an ACQUITY CM-A to use four 50-mm columns - WKB122569

OBJECTIVE or GOAL

Make the appropriate fluidics connections to use four 50-mm columns in the ACQUITY CM-A.

Configure for same in the ACQUITY console.

ENVIRONMENT

• ACQUITY UPLC Column Manager CM-A
• ACQUITY UPLC H-Class
• ACQUITY UPLC I-Class

PROCEDURE

1. Review the tubing configuration found in the ACQUITY CM-A Overview and Maintenance Guide (in version 02 of the document, on page 50) and implement.
2. To configure in the software, go to the ACQUITY Console and open the section for the Column Manager.
3. Click the Configure menu and choose "Instrument".
4. In the box that appears (see picture), change from 2 columns to 4 columns.

5.  Configure the column designations as required and click OK.

ADDITIONAL INFORMATION

Requires kit ACQUITY UPLC 4 x 50mm CM-A (205001832) for the tubing to make the connections.

An ACQUITY BIO configuration requires kit ACQUITY UPLC 4 x 50mm CM-A MP35N (205001830) instead of the regular 316 Stainless Steel kit.

Four-column configuration in the CM-A is possible with the following constraints: Length of column = 50 mm maximum; column ID = 4.6 mm maximum; no column guard or column in-line filter