Progenesis QI for Proteomics version 3 MSe processing hanging - WKB55784
Article number: 55784
SYMPTOMS
- MSe, HD-MSe or SONAR data processing hangs Progenesis QI for Proteomics version 3
- All the RAM on the computer is being used and so the computer is generally slow and unsresponsive
- If you open the Windows Task manager, go the Process tab and sort by RAM usage a process called peptide3D.exe is using almost all the available RAM
ENVIRONMENT
- Progenesis QI for Proteomics version 3
NOTE - this is specific to version 3. It is corrected in version 4 and later
CAUSE
The default low energy to high energy peak matching settings used by the peptide3D.exe during MSe processing mean peptide3D requires a lot of RAM, particularly if the Apex3D thresholds are too low due to not having been optimised correctly. As a result even a PC with 64GB of RAM may struggle with some data sets. These settings are not configurable from within Progenesis QI for Proteomics, but they can be modified using a command file.
FIX or WORKAROUND
- Save the attached file peptide3D_params.txt to C:\Program Files (x86)\Nonlinear Dynamics\Progenesis QI for proteomics\Plugins\WatersRawFolderReader\Processing\
- Optimise the Apex3D thresholds for the data being processed (see WKB1232) and reprocess the data.
ADDITIONAL INFORMATION
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